PROCEDURE
As both good surgical practice, and out of respect for our donors, leave regions of the body covered when they are not part of the day's dissection.
You are expected to understand the anatomy of both sexes. At several points in the dissection instructions, you will be instructed to swap with a nearby table to review examination steps so that you have the opportunity to examine both sexes. You can look back and forth with the nearby tables more often as the groups prefer.
Take a moment to
review the laboratory map which indicates the nearby table with which to swap to
view a donor of the opposite sex.
--------> click here
for (Laboratory
Swap Map).
There may not be an 1:1 ratio of male to female in your quadrant, thus some tables will swap over to another quadrant or need to coordinate with a second group at one table.
The instruction set for male or female will be longer or shorter at points during the various labs across the curriculum. So if your identified swap table isn't ready on a given day, be patient and don't overly pressure them. The converse timing mismatch may happen to your table at some point.
You might find it useful to perform review study until the other table is ready for a swap.
Introductory Video Guides (pre-work before lab)
Sharps Safety
Tools #1 - Scalpel Safety (2min)
Tools #2 - Scalpel Storage (30sec)
Tools #3 - Scalpel Disposal (30sec)
CAUTION: Scalpels are very sharp instruments and must be handled with care following established safety procedures as shown in the videos.
The safest way to keep track of scalpels during a lab is to place all scalpels into an Emesis basin when not in use. These here are several stacked kidney shaped Emesis basins in the table drawers for this purpose.
Foundation Dissection Skills
Removing Skin & Superficial Fascia (3min)
Removing Deep Fascia From Muscle (3min)
Reflecting/Retracting Muscles (3min)
Exposing Veins, Arteries, and Nerves (3min)
Identifying Veins, Arteries, and Nerves (6min)
1. PRE-WORK
(before lab) review the organization of the bony thoracic cage and pelvis from your atlas
or on an articulated skeleton in the lab (these are the same structures reviewed
in the previous session).
(Figure240a)
(Figure201) (Netter187)(Netter192)
a) Sternum: Identify the sternal (jugular) notch, manubrium, body of the sternum, and xiphoid process.
b) Sternal Joints: The manubrium and body of the sternum are connected by the manubriosternal joint (also known as the 'sternal angle').
This is a cartilaginous joint with little to no movement possible. The two bones make an 'angle' of ~160-170 degrees forming a palpable ridge. The body of the sternum and xiphoid process are also connected by a cartilaginous joint (the xiphisternal joint) which has more movement than the manubriosternal joint.
c) Rib cage: Identify the inferior edge of the rib cage, the costal margin, noting the anteroinferior slope of the ribs (i.e. they start higher in the back and curve around to the front). The ribs make a sharp change in curvature (a rotation) at the lateral sides of the thoracic cage. This change in curvature is the costal angle (or angle of the rib).
Although rib fracture can occur anywhere, the weakest part of the rib is just anterior to the costal angle (i.e. posterolateral on the thorax). Thus, this is a common location for rib fracture. Simple rib fractures rarely require any intervention or treatment beyond reduced physical activity of the patient.
d) Clavicle: Identify the clavicle, which extends from the superolateral aspect of the sternum (sternal end of the clavicle) lateral out to the shoulder. The lateral end of the clavicle connects to the acromion (on the scapula). The medial end of the clavicle attaches to the sternum just lateral to the jugular notch at the sternoclavicular joint.
e) Rib articulations: Sternocostal joints attach all the ribs to the sternum via their costal cartilage. Ribs 1-7 attach directly to the sternum while ribs 8-10 share a common cartilaginous attachment to the sternum.
Radiology - Review the landmarks above and from the prior lab for how they appear within a CTX. (Rad2001)
CLINICAL EXERCISE - Tube Thoracostomy
2. Interventions through the thoracic wall to gain access to the lung spaces are common lifesaving procedures following lung collapse. The concept of chest drainage dates back to Hippocrates himself who described a drainage procedure using metal tubes.
In this clinical exercise, you have the chance to insert a chest tube to your donor in a mock surgical intervention.
For the steps to
take:
------
click here
------
3. Before cutting into the skin, review the markings on your donor from the prior lab session
as we will use these as part of the planned incision lines.
(Figure242)
(Rad1001)
The incision plan will use your previously marked midsternal, clavicle, axilla, and costal margin landmark lines. These incision marks demarcate a left and right anterior thorax skin flap which will be reflected in the following steps.
Successful outcome of surgical procedures is, logically, strongly correlated with the surgeon getting the initial incisions in the correct place.
4. Start at the jugular notch following your incision
marking, make a deep 'to-the-bone'
incision in the skin at the midline extending the full length of the
anterior surface of the sternum.
(Figure243)
Use the traction and counter-traction method from the skin incision foundational skills video in the pre-lab section above.
This is a safe zone for a deep incision as there are no nerves or vessels crossing from the left to right side at the midline and the sternum protects thoracic structures from the incision.
If you have not already reviewed the video on skin incisions, take a moment to review the video instruction section with this link. ---- click here ---->
5. Make a second incision several millimeters in depth following
the full length of the clavicular
incision marking so that you now have a T shaped incision.
(Figure243a)
The traction and counter-traction method results in the incision opening slightly during cutting allowing a visualization of the depth of the cut. The cut can always be deepened later if the initial incision is too shallow.
6. Make a third incision quiet shallow in depth
following just superior to the costal margin incision marking all the
way to the mid-axillary line.
(Figure243b)
Make this incision shallow and ensure that the incision is superior to the costal margin.
It is important not to cut through the anterior abdominal wall. Thus, by making the cut just superior to the costal margin the abdomen is protected against cutting too deeply.
7. Make a fourth incision several millimeters in depth
following the deltopectoral groove marking on the anterior thorax.
(Figure243c)
Avoid cutting too deeply along this line as excessive depth may transect part of the pectoralis major muscle. A shallow cut can always be deepened later.
7. Using a hemostat (locking forceps), at the superior end
of the midsternal incision clamp the skin at
the corner where the incision lines intersect and pull the corner of skin, so that
you can make small incisions under the lifted edge to reflect the skin.
(Figure244)
If you have not already reviewed the video on skin reflection, take a moment to review the video instruction section with this link. ---- click here ---->
8. Widen and deepen the cut until you reach
the plane between the superficial (hypodermis) and deep fascia covering the
muscles.
(Figure103)
Fascia and any other human tissues removed from your donor should be placed into one of your Emerson basins for disposal in the marked human-tissue biohazard containers (these are by the lab central pillars). There is already a biohazard bag liner in the bin so you do not need to place tissues for disposal into a separate biohazard bag. Do not place human tissues into the regular trash or the glove disposal boxes.
At this point you can observe the depth of the cut and identify the layers of the integument (skin). From superficial to deep these layers are the epidermis, dermis, and hypodermis.
The mammary gland tissue lies in the superficial (hypodermis) fascia. (Netter188)
Epidermis: The epidermis is only a few hundred micrometers in thickness and will exhibit different degrees of pigmentation depending on the individual.
Dermis: The dermis is a stronger layer of connective tissue (dense irregular connective tissue) up to several millimeters thick that is fused tightly to the epidermis. To the eye, the dermis typically exhibits a relatively uniform pale color.
Hypodermis: The hypodermis varies widely in thickness depending on the region of the body and body habitus of the individual. At the midline it may range from a few millimeters up to a centimeter while thickness in the lower torso can be several inches.
The hypodermis in a well nourished individual will have yellow adipose connective tissue regions interspersed with fibrous connective tissue. Within the hypodermis are numerous small blood vessels and nerves (most of these are microscopic in size).
The deep or investing fascia is not considered part of the integument organ (i.e. the skin). Deep fascia surrounds and encases muscles of the body (thus the alternate term 'investing' fascia).
On the torso, the deep fascia layer lies immediately below the hypodermis and directly on the surface of the pectoralis major muscle.
Deep fascia is a very thin layer that typically has a white smooth membranous appearance. Muscle tissue underneath the deep fascia is darker and has a distinct appearance consisting of parallel fascicles.
Each of these fascicles is formed by bundles of individual muscle cells. On the torso, where you are dissecting, these muscle fascicles belong to the pectoralis major muscle (we will examine the pectoralis major muscle in detail later in the lab).
9. Use your hemostats to apply continuous tension as you
retract the skin and separate along the plane between the superficial fascia
and the deep fascia.
(Figure244a)
If you find yourself cutting into muscle tissue you are dissecting too deeply and need to move shallow back to the correct plane.
Stay along the surface of the muscle (deep fascia) so as to be able to preserve the breast tissue intact.
Hold the scalpel at an angle and use smooth slicing motions to cut with the tip of the blade. Each cut should aim to separate a few millimeters of tissue, allowing you to observe what you are cutting while making the incisions.
Sawing motions are to be avoided. Sawing has less control and can result in jagged edge cuts that, in the living, may not heal as well as smooth cuts. Commonly in surgery we would use electrosurgical cutters that simultaneously cut and cauterize.
In some regions pushing your fingers into the plane between the superficial and deep fascia will separate the tissue along the fascial plane.
It may help to have a member of the team keep tension on the skin while a second member is cutting. Once there is room to work safely, dissection can proceed simultaneously on the left and right side of the donor.
10. Reflect the skin following along the superior and inferior
incision lines out to the mid-axillary line.
(Figure244b)
As you reflect the skin take care to follow the plane between the superficial fascia (hypodermis layer) and the deep fascia covering the chest plate and the pectoralis major muscle such that you can reflect both breasts and surrounding integument.
The breast and surrounding skin area should be free of any attachments along the medial, superior and inferior regions, with the only attachment along the lateral edge at the mid-axillary line.
11. Place the breast flap back into position and on only
one of the breasts make a parasagittal cut through the nipple and areola several
inches in length.
(Figure204)
(Photo2072)
Recall, a parasagittal incision is one that runs superior to inferior, or if using the clock facer nomenclature of the breast from 12 o'clock to 6 o'clock.
If you had identified and marked a possible breast mass or region of concern, then change the axis of the incision such that it passes through the region of concern.
This will allow examination of the mass and potential biopsy of the tissue for pathology assessment.
12. Use a Weitlaner retractor to spread the skin incision
to observe the exposed surface of the hemisected breast.
(Photo2073)
The exposed surface of breast will have adipose tissue (yellow) and pale fibrous septa of the suspensory ligaments of the breast (also known as Cooper's ligaments).
13. With a blunt probe remove some of the adipose tissue
and observe that the suspensory ligaments of the breast form a honeycomb like
arrangement of fibrous sheets.
(Figure211) (Netter188) (Photo2074)
The suspensory ligaments of the breast serve a structural support function, tethering the skin of the breast to the deep fascia and supporting both glandular and adipose tissue of the mammary gland.
In the male, suspensory ligaments of the breast are absent or rudimentary in structure.
14. Probe the connective tissue near the nipple and note
the appearance of 'striations' representing the
terminal elements of the breast duct system, the lactiferous ducts and sinuses.
(Figure211)
(Figure212) (Netter188)
(Photo2075)
In males, ducts from the nipple are very short and rarely extend beyond the boundary of the areola.
In females, a system of ducts extends from the nipple into the surrounding stroma (body) of the breast. This system of ducts in the aged breast is less well defined than in a younger individual, as they undergo an involution process with age.
Any striation line or strand like structure from the nipple will be a remnant of the lactiferous duct system.
The female breast is divided into 15-20 irregular lobes of glandular tissue. The glandular tissue of each lobe leads to a single lactiferous duct terminating in a small swelling, a lactiferous sinus, just before openings at the nipple.
Lobes are organized in a roughly radial manner around the nipple. However, it is not possible to visualize a lobe clearly as duct branches can cross and interweave with those of an adjacent lobe.
15. When you have completed the examination of the breast of your donor you will swap tables briefly so that you can examine the breast of the opposite sex.
Remember to review the Patient chart at your swap table so that you know the name of the donor and any clinical notes entered by the other team before your examination them.
SWAP POINT: At this point, swap with your
pre-identified table containing a donor of the opposite sex and go
through the steps above examining the breast structure. Then return to your donor to continue steps
below.
--------> click here for (Laboratory
Swap Map).
16. When you have finished examination of the other table, return to your table and remove the Weitlaner retractor.
17. Reflect the thoracic skin flaps laterally so that you can observe the surface of the pectoralis major muscle.
18. Clean the surface of the pectoralis major muscle and observe
its clavicular, sternal, and costal attachments.
(Netter194)
(Photo2001)
The pectoralis major muscle forms up the bulk of the chest musculature with the breast positioned anterior to the muscle anchored to the pectoral fascia (deep fascia on the surface of pectoralis major).
19. Force your fingers deep to pectoralis major along its
inferior border (about the 4th or 5th rib on the lateral chest wall) and mobilize the inferior edge by lifting superiorly.
(Figure205)
(Photo2076)
The inferior edge is frequently tightly bound in fascia. Thus, you may need to make a small scalpel cut to start the opening under the muscle edge.
Do not cut near the costal margin as you may damage abdominal wall musculature that we will be studying in a later lab session.
20. Sever the pectoralis major muscle costal attachment, the sternal
attachment, and approximately halfway along the clavicular attachment.
(Figure205,
cut lines) (Photo2077)
(Photo2078)
21. Reflect the pectoralis major muscle laterally and superiorly, using fingers to
gently separate the muscle from
underlying structures.
(Figure206a)
(Photo2079)
On the deep surface of the pectoralis major muscle the medial pectoral nerve enters the muscle. If you are gentle in separating and reflecting the muscle, your fingers will find a tough 'strand' or 'string' like structure which is the nerve.
Under the superior edge of pectoralis major, near the clavicle, are additional nerves and vessels entering the muscle (you saw the location of these in the introductory techniques videos). We will not dissect this area today, as that is part of another session.
22. Fully reflect the pectoralis major muscle and observe the
underlying pectoralis minor muscle.
(Figure206a)
(Netter195)
(Photo2080)
As you uncover pectoralis minor, if you found the medial pectoral nerve trace it proximally and observe that the nerve pierces through the pectoralis minor muscle before entering the pectoralis major muscle.
23. When you have finished the laboratory dissection for today, return the reflected pectoralis muscles to their correct position and return the reflected breast skin regions back into position covering the thorax.
DONOR CARE
Then proceed with donor care, covering the donor with the moist cloth, closing the inner plastic liner and securing with several hemostats, zipping up the white plastic outer bag, and neatly covering the donor with the blue drape.
Remember to check the drain bucket under your table and if needed follow the emptying procedures from the previous lab (first fetch an empty bucket from the shelving near the fluid disposal drums, swap out your bucket for the empty bucket, then empty your bucket into the drum and leave the now empty bucket on top of the drum for the next group).
CHECKLIST
Skeletal Structures
Sternum
Manubrium
Body of the sternum
Xiphoid process
Sternal (jugular) notch
Sternoclavicular joint
Ribs
Costal margin
Sternocostal joints
Soft Structures
Breast
Nipple
Areola
Suspensory (
Lactiferous ducts
Pectoralis major